The 50:50 method for PCR-based seamless genome editing in yeast
نویسندگان
چکیده
منابع مشابه
A CRISPR/Cas9-based method and primer design tool for seamless genome editing in fission yeast
In the fission yeast Schizosaccharomyces pombe the prevailing approach for gene manipulations is based on homologous recombination of a PCR product that contains genomic target sequences and a selectable marker. The CRISPR/Cas9 system has recently been implemented in fission yeast, which allows for seamless genome editing without integration of a selection marker or leaving any other genomic 's...
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In the fission yeast the prevailing approach for Schizosaccharomyces pombe gene manipulations is based on homologous recombination of a PCR product that contains genomic target sequences and a selectable marker. The CRISPR/Cas9 system has recently been implemented in fission yeast, which allows for seamless genome editing without integration of a selection marker or leaving any other genomic ‘s...
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ژورنال
عنوان ژورنال: Yeast
سال: 2013
ISSN: 0749-503X
DOI: 10.1002/yea.2992